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Published on: July 6, 2016
Insulator base pairs for lighting-up perylenediimide in a DNA duplex
Hiromu Kashida1, Koji Sekiguchi, Hiroyuki Asanuma
1Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Abstract:
Quenched: Perylenediimide (PDI) is highly quenched by nucleobases, which greatly restricts its application as a fluorescent probe. Here, we propose "insulator base pairs" tethering cyclohexane ring through D-threoninol. When "insulator base pairs" were inserted between PDI and nucleobases, the quantum yield of PDI drastically increased several thousand-fold. The "insulator base pairs" reported here also have the potential to increase the quantum yields of other fluorophores.
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