Molecular spectroscopic analysis of nano-chitosan blend as biosensor

Chitosan/starch and chitosan/gelatin of different ratios were prepared following casting method. FTIR results indicate the formation of hydrogen bonding which dedicates the prepared blends for interaction with wide range of molecules specially those of NH₂ and COOH terminals. The results obtained with molecular modeling PM3 model are in agreement with spectroscopic data. As a result of increasing starch and gelatin in chitosan blends HOMO-LUMO energy slightly decreased while total dipole moment increased. UV-vis spectroscopy indicated the suitability of chitosan/starch blend as a glycine sensor. Further enhancement in the sensing performance of chitosan/starch blend was achieved by introducing 5 nm TiO₂ into the blend.