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Published on: November 5, 2010
Quantification of the hematoporphyrin derivative by fluorescence measurementusing dual-wavelength excitation
Abstract:
A theory is presented describing a method for quantitative measurement of fluorophores in vivo. The method employs a combination of two-wavelength excitation and two-wavelength detection. A dimensionless fluorescence ratio is defined. The theory predicts that this ratio is a function of the concentration of the fluorophore only and is independent of optical properties, independent of geometrical parameters, and independent of autofluorescence. Experiments using a O.1-10-microg/mL hematoporphyrin derivative in a tissue phantom with the optical properties of the dermis show good agreement with theory.
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