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Updated: Jun 9, 2026
Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
Published on: July 20, 2019
Tumor growth and angiogenesis is impaired in CIB1 knockout mice
Mohamed A Zayed1, Weiping Yuan, Dan Chalothorn
1Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. parise@med.unc.edu.
Background:
Pathological angiogenesis contributes to various ocular, malignant, and inflammatory disorders, emphasizing the need to understand this process more precisely on a molecular level. Previously we found that CIB1, a 22 kDa regulatory protein, plays a critical role in endothelial cell function, angiogenic growth factor-mediated cellular functions, PAK1 activation, MMP-2 expression, and in vivo ischemia-induced angiogenesis. Since pathological angiogenesis is highly dependent on many of these same processes, we hypothesized that CIB1 may also regulate tumor-induced angiogenesis.
Methods:
To test this hypothesis, we allografted either murine B16 melanoma or Lewis lung carcinoma cells into WT and CIB1-KO mice, and monitored tumor growth, morphology, histology, and intra-tumoral microvessel density.
Results:
Allografted melanoma tumors that developed in CIB1-KO mice were smaller in volume, had a distinct necrotic appearance, and had significantly less intra-tumoral microvessel density. Similarly, allografted Lewis lung carcinoma tumors in CIB1-KO mice were smaller in volume and mass, and appeared to have decreased perfusion. Intra-tumoral hemorrhage, necrosis, and perivascular fibrosis were also increased in tumors that developed in CIB1-KO mice.
Conclusions:
These findings suggest that, in addition to its other functions, CIB1 plays a critical role in facilitating tumor growth and tumor-induced angiogenesis.
Insights
The protein CIB1 (cellular inhibitor of apoptosis protein 1) is crucial for tumor growth and angiogenesis. Mice lacking CIB1 showed reduced tumor size and decreased blood vessel formation, highlighting CIB1
Area of Science:
- Molecular biology
- Cancer research
- Angiogenesis research
Background:
- Pathological angiogenesis is implicated in ocular, malignant, and inflammatory diseases.
- CIB1 (cellular inhibitor of apoptosis protein 1) is a regulatory protein vital for endothelial cell function and angiogenesis.
- CIB1's role in angiogenesis suggests a potential involvement in tumor-induced angiogenesis.
Purpose of the Study:
- To investigate the role of CIB1 in tumor growth and angiogenesis.
- To test the hypothesis that CIB1 regulates tumor-induced angiogenesis.
Main Methods:
- Allografting murine B16 melanoma or Lewis lung carcinoma cells into wild-type (WT) and CIB1-knockout (CIB1-KO) mice.
- Monitoring tumor growth, morphology, histology, and intra-tumoral microvessel density.
Main Results:
- Melanoma tumors in CIB1-KO mice were smaller, necrotic, and had reduced microvessel density.
- Lewis lung carcinoma tumors in CIB1-KO mice exhibited smaller volume and mass, with decreased perfusion.
- Increased intra-tumoral hemorrhage, necrosis, and perivascular fibrosis were observed in tumors from CIB1-KO mice.
Conclusions:
- CIB1 plays a critical role in facilitating tumor growth.
- CIB1 is essential for tumor-induced angiogenesis.
- These findings underscore CIB1's significance in cancer progression.
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