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Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
Published on: October 17, 2022
Acoustophoresis in shallow microchannels
Mehti Koklu1, Ahmet Can Sabuncu, Ali Beskok
1Department of Mechanical and Aerospace Engineering, Old Dominion University, Norfolk, VA 23529, USA.
Journal of Colloid and Interface Science
|September 1, 2010
Summary
Acoustophoretic motion in microchannels was studied. Particle depth and acoustophoretic force were accurately predicted, crucial for designing micro sample concentration devices.
Area of Science:
- Fluid dynamics
- Acoustic manipulation
- Microfluidics
Background:
- Acoustophoretic (AP) manipulation is a key technique in microfluidics.
- Understanding particle behavior in shallow microchannels is essential for device design.
- Wall effects significantly influence particle dynamics in confined geometries.
Purpose of the Study:
- Investigate acoustophoretic motion of polystyrene particles in shallow microchannels.
- Predict particle depths and extract AP forces experimentally.
- Analyze the impact of wall effects on AP force and particle focusing.
Main Methods:
- Utilized particle tracking to analyze trajectories in a pressure-driven flow.
- Employed second-order perturbation theory to explain particle migration.
- Applied the particle equation of motion to determine AP forces, including wall corrections.
Main Results:
- Particle depths were influenced by ultrasonic actuation voltage.
- Wall corrections led to significant (up to 20%) differences in AP force magnitude.
- Calculated focal length showed good agreement with experimental results.
Conclusions:
- Experimental and theoretical AP force predictions align well.
- The focal length is a critical parameter for designing effective micro sample concentrators.
- This study provides valuable insights into particle behavior for microfluidic applications.

