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Updated: Jun 9, 2026

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
Published on: September 6, 2011
Fabrication of complex patterns with a wide range of feature sizes from a single line prepattern by successive
Su-Kyong Lee1, Jin-Mi Jung, Ji-Sun Lee
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Korea.
Abstract:
The variously shaped gold patterns can be generated from the polydimethylsiloxane (PDMS) mold using line patterns by the capillary force lithography (CFL) process, which is a kind of nanoimprint method following the two-cycle method. After fabrication of micro- or nanosized line patterns at the first cycle, the patterned substrate is used as a substrate for the second cycle of CFL. When the other stamp is placed on the first pattern, rotated by a certain angle with respect to the first stamp, only the overlapped parts remained dot-shaped after the etching process. The various shapes and sizes of patterns can be produced by controlling the CFL conditions such as polymer thickness, reactive ion etching (RIE) time, and degree of rotation angle. The key advantage of the double imprint lithography method is to get the nanosized isolated dot-shaped patterns from microsized line patterns. If we fabricate nanosized isolated dot-shaped patterns directly, we should need predesigned patterns in the form of a master, which is generally prepared by a high-cost and time-consuming process such as E-beam lithography. The successful applications of large-area periodic patterns are nanoelectronic devices, nanoelectromechanical system (NEMS), and biosensors, the template of which is the master of nanoimprint lithography (NIL) and stamp fabrication in soft lithography.

