Jove
Visualize
Contact Us

Related Experiment Videos

Multiple forms of transketolase.

A Kuimov1, M Filippov, G Kochetov

  • 1Department of Animal Cell Biochemistry, A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.

Biochemistry International
|September 1, 1990
PubMed
Summary

Transketolase exists in multiple forms with varying thermostability. These enzyme forms share similar characteristics across different species, suggesting physiological state influences their ratio.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drosophila RNase H1 is essential for development but not for proliferation.

Molecular genetics and genomics : MGG·2001
Same author

Localization of reactive tyrosine residues of baker's yeast transketolase.

FEBS letters·1996
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Biochemistry
  • Enzymology

Background:

  • Transketolase is a key enzyme in the pentose phosphate pathway.
  • Enzyme multiplicity and isoforms are common in biological systems.

Purpose of the Study:

  • To investigate the multiplicity and characteristics of transketolase forms.
  • To compare transketolase forms across different species and tissues.

Main Methods:

  • Phosphocellulose chromatography for enzyme separation.
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for mobility analysis.
  • Thermostability assays.

Main Results:

  • Multiple transketolase forms with differing thermostability were identified.
  • Yeast transketolase forms exhibited identical mobility via SDS-PAGE.
  • Transketolase forms from yeast, rat liver, pig liver, and rabbit organs showed similar chromatographic behavior and thermostability.
  • The ratio of transketolase forms appears dependent on the organism's physiological state.

Conclusions:

  • Transketolase exhibits molecular heterogeneity with conserved properties across species.
  • The physiological state significantly influences the expression and ratio of transketolase isoforms.

Related Experiment Videos