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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
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Published on: October 23, 2011

A multiplex nanoparticle-based bio-barcoded DNA sensor for the simultaneous detection of multiple pathogens.

Deng Zhang1, Michael C Huarng, Evangelyn C Alocilja

  • 1Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, United States.

Biosensors & Bioelectronics
|September 3, 2010
PubMed
Summary

This study presents a novel nanoparticle biosensor for detecting Bacillus anthracis and Salmonella enteritidis DNA simultaneously. The highly amplified electrochemical system offers sensitive detection of pathogenic genes in a single sample.

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Area of Science:

  • Nanotechnology
  • Biosensors
  • Electrochemistry
  • Molecular Diagnostics

Background:

  • Simultaneous detection of multiple pathogenic agents is crucial for public health.
  • Existing methods for detecting Bacillus anthracis and Salmonella enteritidis can be time-consuming and require separate assays.
  • Nanoparticle-based biosensors offer potential for enhanced sensitivity and multiplexing capabilities.

Purpose of the Study:

  • To develop a highly amplified, nanoparticle-based, bio-barcoded electrochemical biosensor.
  • To achieve simultaneous multiple detection of the Bacillus anthracis pagA gene and the Salmonella enteritidis Iel gene.
  • To evaluate the sensitivity and potential applications of the developed biosensor system.

Main Methods:

  • Fabrication of a multiplex biosensor using gold nanoparticles (AuNPs), magnetic nanoparticles (MNPs), and nanoparticle tracers (NTs).
  • Design of target-specific DNA probes (1pDNA and 2pDNA) for capturing target DNA sequences.
  • Formation of a sandwich structure (MNP-2pDNA/tDNA/1pDNA-AuNP-bDNA-NTs) followed by magnetic separation.
  • Electrochemical detection of dissolved NT(2+) ions using square wave anodic stripping voltammetry (SWASV) on screen-printed carbon electrode (SPCE) chips.

Main Results:

  • The biosensor achieved simultaneous detection of both target genes.
  • Detection limits were as low as 0.5 ng/mL for the Salmonella enteritidis Iel gene (using CdS NTs) and 50 pg/mL for the Bacillus anthracis pagA gene (using PbS NTs).
  • The system demonstrated significant signal amplification due to multiple NTs per binding event.

Conclusions:

  • The developed nanoparticle-based bio-barcoded electrochemical biosensor enables sensitive and simultaneous detection of multiple pathogenic DNA targets.
  • The high amplification and multiplexing capability suggest potential for rapid detection of various pathogens in a single sample.
  • This technology holds promise for applications in food safety, clinical diagnostics, and biodefense.