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Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
Published on: November 26, 2011
Analysis of MAP kinases by hydrogen exchange mass spectrometry
1Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2010
Abstract:
Hydrogen exchange mass spectrometry (HX-MS) is an experimental technique that can be used to -examine solvent accessibility and conformational mobility in biological macromolecules. This chapter summarizes studies using HX-MS to examine the regulation of conformation, protein mobility, and ligand binding to MAP kinases. We describe the planning and design of HX-MS experiments, strategies for data analysis and interpretation, and available software.
