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Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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Interactions Between Signaling Pathways

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Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
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In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

MAP Kinase: SUMO pathway interactions.

Shen-Hsi Yang1, Andrew D Sharrocks

  • 1Faculty of Life Sciences, University of Manchester, Manchester, UK.

Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2010
PubMed
Summary
This summary is machine-generated.

This study details protocols for investigating the cross-talk between Mitogen-Activated Protein Kinase (MAPK) and SUMOylation pathways. Understanding this interaction is crucial for deciphering gene regulation in response to environmental signals.

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Last Updated: Jun 9, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

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Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
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Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Gene Regulation

Background:

  • Cellular responses to environmental cues are governed by complex signaling networks.
  • Mitogen-Activated Protein Kinase (MAPK) pathways are key mediators of extracellular signals.
  • Protein SUMOylation (Small Ubiquitin-like Modifier) is a critical post-translational modification regulating diverse cellular processes.

Purpose of the Study:

  • To describe protocols for studying the cross-talk between MAPK and SUMOylation pathways.
  • To investigate how these pathways converge to modulate transcription factor activity.
  • To provide insights into the regulation of gene expression via post-translational modifications.

Main Methods:

  • Development and description of experimental protocols.
  • Focus on studying the interplay between MAPK signaling and protein SUMOylation.
  • Analysis at the level of gene regulation.

Main Results:

  • Established links between MAPK pathway activation and de-SUMOylation of transcription factors (e.g., Elk-1).
  • Demonstrated that MAPK pathways influence protein SUMOylation, with effects varying across different proteins.
  • Highlighted the convergence of SUMO and MAPK pathways in modulating transcription factor activity.

Conclusions:

  • The cross-talk between MAPK and SUMOylation pathways is significant for cellular signaling and gene regulation.
  • Understanding these interactions provides a framework for studying complex biological outcomes.
  • The described protocols facilitate further research into this critical regulatory network.