Related Experiment Video
Updated: May 6, 2026
11:13
Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
7.8K
The ATAC acetyltransferase complex coordinates MAP kinases to regulate JNK target genes
Tamaki Suganuma1, Arcady Mushegian, Selene K Swanson
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
Cell
|September 4, 2010
Summary
The ATAC histone acetyltransferase complex acts as a cofactor for c-Jun, regulating gene expression and suppressing upstream signaling during the cellular response to stress.
Area of Science:
- Molecular Biology
- Cell Signaling
- Epigenetics
Background:
- Signal transduction pathways, such as Jun N-terminal kinase (JNK), activate transcription factors like c-Jun to regulate gene expression in response to extracellular stimuli.
- Histone acetyltransferase (HAT) complexes play crucial roles in modulating chromatin structure and influencing gene transcription.
Purpose of the Study:
- To investigate the role of the ATAC (Ada two A containing) histone acetyltransferase complex as a transcriptional cofactor for c-Jun.
- To elucidate the function of ATAC in regulating JNK target genes and its involvement in the cellular stress response.
Main Methods:
- Chromatin immunoprecipitation assays to assess c-Jun occupancy and H4K16 acetylation at target genes.
- Immunofluorescence microscopy to observe the colocalization of ATAC and c-Jun under osmotic stress.
- Biochemical assays to analyze the recruitment of kinases and the suppression of JNK activation.
Main Results:
- ATAC complex is essential for c-Jun binding to Jra and chickadee genes and for H4K16 acetylation.
- ATAC colocalizes with c-Jun during osmotic stress, recruiting upstream kinases (Misshapen, MKK4, JNK) and inhibiting further JNK activation.
- The CG10238 subunit of ATAC is critical for MAPK relocalization and JNK pathway suppression.
Conclusions:
- The ATAC complex functions as a transcriptional coactivator for c-Jun at specific target genes.
- ATAC acts as a negative regulator of upstream MAP kinase signaling, suppressing JNK activation during stress.
- ATAC integrates transcriptional regulation and signal transduction modulation in response to cellular cues.
More Related Videos
09:32Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
8.0K
07:38Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
Published on: June 6, 2025
959
Related Concept Videos
01:42Microtubule Associated Proteins (MAPs)
4.8K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
4.8K
01:19Interactions Between Signaling Pathways
4.7K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
4.7K
01:07MAPK Signaling Cascades
7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
01:20The JAK-STAT Signaling Pathway
10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
10.2K
01:22PI3K/mTOR/AKT Signaling Pathway
5.2K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.2K
01:25cAMP-dependent Protein Kinase Pathways
7.3K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
7.3K