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[HPLC fingerprint spectrum of honey-fried Radix Glycyrrhizae]
Objective:
To establish the HPLC fingerprint of the pieces of honey-fried Radix Glycyrrhizae.
Method:
Using the reverse-performance liquid chromatography, method was performed on a Hyperclone ODS C18 column (4.6 mm x 250 mm, 5 microm) and acetonitrile-0.1% phosphoric acid was selected as mobile phase gradient elution were adopted.
Result:
Established HPLC fingerprint of Radix et Rhizoma glycyrrhizae pieces were established, and the results of methodological study met the technical requirements for fingerprinting.
Conclusion:
The HPLC method is stable, accurate, and reliable to provide a scientific basis of quality control standard for the honey-fried Radix et Rhizoma glycyrrhizae.
