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Updated: Jun 9, 2026

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Published on: May 15, 2019
[Scl1 of M41-type group A streptococcus binds low-density lipoprotein]
Chunwei Liang1, Ruidong Zhao, Yuming Gao
1College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China. chunweiliang@yahoo.cn
Objective:
To study the interaction of streptococcal collagen-like protein 1 (Scl1) of M41-type group A streptococcus (GAS) ATCC12373 with low-density lipoprotein (LDL).
Methods:
We cloned, expressed and purified the recombinant proteins rScl1 and its V region rScl1-V, designated as C176 and C176V, derived from Scl1. 41 of M41-type GAS. The binding of rScl1 to LDL was detected with affinity chromatography-binding assay, Western blot analysis and enzyme-linked immunosorbent assay (ELISA), and whole cell binding assays were used to detect the interactions of whole GAS cells with LDL.
Results:
The results demonstrated that C176 and C176V could specifically bind purified LDL, and M41-type GAS cells expressing native Sell could bind LDL whereas M6-type GAS could not.
Conclusion:
The Sell of M41-type GAS specifically binds LDL.
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