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Published on: May 15, 2019
[Scl1 of M41-type group A streptococcus binds low-density lipoprotein]
Chunwei Liang1, Ruidong Zhao, Yuming Gao
1College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China. chunweiliang@yahoo.cn
Streptococcal collagen-like protein 1 (Scl1) from M41-type group A Streptococcus specifically binds low-density lipoprotein (LDL). This interaction was confirmed using recombinant proteins and whole bacterial cells, highlighting a unique bacterial-host molecule interaction.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Context:
- Group A Streptococcus (GAS) possesses surface proteins that mediate host interactions.
- M41-type GAS expresses streptococcal collagen-like protein 1 (Scl1), a key virulence factor.
- Low-density lipoprotein (LDL) is a crucial host molecule involved in various physiological and pathological processes.
Purpose:
- To investigate the binding interaction between Scl1 from M41-type GAS and LDL.
- To determine if Scl1 mediates the binding of GAS to LDL.
Summary:
- Recombinant Scl1 (rScl1) and its V region (rScl1-V) were expressed and purified.
- Affinity chromatography, Western blot, and ELISA confirmed specific binding of rScl1 to purified LDL.
- Whole M41-type GAS cells expressing Scl1 bound LDL, while M6-type GAS cells did not.
Impact:
- Establishes a specific molecular interaction between Scl1 and LDL.
- Suggests a mechanism for M41-type GAS adherence or modulation of host lipid metabolism.
- Provides a basis for further research into the role of Scl1-LDL interaction in GAS pathogenesis.
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