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Related Concept Videos

Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

Transcript analysis of stem cells.

Alison V Nairn1, Mitche dela Rosa, Kelley W Moremen

  • 1The Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.

Methods in Enzymology
|September 7, 2010
PubMed
Summary

Quantitative real-time polymerase chain reaction (qRT-PCR) offers flexible transcript abundance analysis. This method aids in understanding glycosylation regulation when combined with glycan and protein data.

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
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Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
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Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) is a versatile technique for measuring gene expression levels.
  • Analyzing transcript abundance is crucial for understanding cellular processes and potential regulatory mechanisms.

Purpose of the Study:

  • To detail the methods for designing and executing qRT-PCR analyses.
  • To illustrate how qRT-PCR data can inform about glycosylation regulation at the transcript level.

Main Methods:

  • Detailed protocols for qRT-PCR experiment design.
  • Step-by-step instructions for performing qRT-PCR assays.
  • Guidance on data acquisition and analysis for transcript abundance.

Main Results:

  • Demonstration of qRT-PCR's scalability from single-gene to high-throughput analysis.
  • Examples of how qRT-PCR results can be integrated with glycan and protein data.
  • Insights into interpreting transcript-level information for glycosylation studies.

Conclusions:

  • qRT-PCR is a powerful tool for transcriptomic analysis with broad applications.
  • The technique provides valuable data for investigating glycosylation regulatory pathways.
  • Effective design and execution of qRT-PCR are key to obtaining meaningful biological insights.