Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ion Exchange

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...

Aquaporins

Aquaporins

Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.

The ADP/ATP Carrier Protein

The ADP/ATP Carrier Protein

ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...

Pore Transport and Ion-Pair Transport

Pore Transport and Ion-Pair Transport

Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct microscopic...

ATP Driven Pumps III: V-type Pumps

ATP Driven Pumps III: V-type Pumps

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Case of Paraplegia from Peripheral Irritation.

The Chicago medical journal·2023

Same author

Case of Wound of the Liver.

The Chicago medical journal·2023

Same author

Tropical distal renal tubular acidosis: clinical and epidemiological studies in 78 patients.

QJM : monthly journal of the Association of Physicians·2012

Same author

Does hypokalaemia cause nephropathy? An observational study of renal function in patients with Bartter or Gitelman syndrome.

QJM : monthly journal of the Association of Physicians·2011

Same author

Review. Leaky Cl--HCO3- exchangers: cation fluxes via modified AE1.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2008

Same author

Acute oxalate nephropathy causing late renal transplant dysfunction due to enteric hyperoxaluria.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2008

Same journal

Corrigendum to "Sodium butyrate down-regulation of indoleamine 2, 3-dioxygenase at the transcriptional and post-transcriptional levels" [Int. J. Biochem. Cell Biol. 42 (2010) 1840-1846].

The international journal of biochemistry & cell biology·2026

Same journal

Whole-brain spatial metabolomics reveals metabolic gradient shifts in a murine glioma model following boron neutron capture therapy (130 characters).

The international journal of biochemistry & cell biology·2026

Same journal

LCN2 modulates Th17/Treg balance in vitro and is associated with an adaptive response to intestinal ischemia-reperfusion injury under Hmox1-deficient conditions.

The international journal of biochemistry & cell biology·2026

Same journal

Chloroquine modulates the redox-sensitive signalling via inhibiting the AMPK-ULK1 under LPS induced state in murine splenic macrophages.

The international journal of biochemistry & cell biology·2026

Same journal

The vicious cycle of hyperglycemia and oxidative stress: Novel mechanistic insights into a pathogenic alliance.

The international journal of biochemistry & cell biology·2026

Same journal

Fibroblast growth factors (FGF) in the pathogenesis and treatment of inflammatory bowel diseases (IBD): An overview.

The international journal of biochemistry & cell biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Functional Characterization of Na⁺/H⁺ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015

Anion exchanger 1: Protean function and associations.

S B Walsh¹, G W Stewart

¹UCL Centre for Nephrology, Royal Free Hospital, University College London, United Kingdom. stephen.walsh@ucl.ac.uk

The International Journal of Biochemistry & Cell Biology

|September 7, 2010

Summary

This summary is machine-generated.

Anion exchanger 1 (AE1), a key erythrocyte membrane protein, has diverse roles beyond chloride-bicarbonate exchange. This review explores its functions in health and disease, including cell volume regulation.

More Related Videos

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Published on: February 19, 2013

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Related Experiment Videos

Last Updated: Jun 9, 2026

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Functional Characterization of Na⁺/H⁺ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Published on: February 19, 2013

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Area of Science:

Biochemistry
Cell Biology
Physiology

Background:

Anion exchanger 1 (AE1) is the most abundant protein in the erythrocyte membrane.
AE1 is also found on the basolateral surface of alpha intercalated cells in the distal nephron.
Mutations in AE1 cause hereditary red cell diseases and distal renal tubular acidosis.

Purpose of the Study:

To review the diverse functions of AE1 beyond its classical role.
To discuss AE1's associations with other membrane proteins and its role in cell volume regulation.
To explore AE1's functions in both health and disease states.

Main Methods:

Literature review of existing research on AE1.
Analysis of AE1's known and emerging functions.
Discussion of AE1's role in various physiological and pathological conditions.

Main Results:

AE1 mediates electroneutral chloride-bicarbonate exchange and contributes to red cell membrane mechanics.
AE1 participates in alternative anion transport, including sulfate transport and proton/sulfate symport.
AE1 associates with other membrane proteins in the AE1 macrocomplex and regulates glycolysis and cation transport.

Conclusions:

AE1 possesses multifaceted roles in erythrocytes and kidney cells, extending beyond anion exchange.
Emerging functions include regulation of glycolysis, cation transport, and cell volume.
Understanding AE1's diverse functions is crucial for comprehending various health and disease conditions.