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Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Granulocyte colony-stimulating factor produces a decrease in IFNgamma and increase in IL-4 when administrated to

Octavio Rodríguez-Cortés1, Jorge Vela-Ojeda, Rubén López-Santiago

  • 1Depto. de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México.

Journal of Clinical Apheresis
|September 7, 2010
PubMed
Summary

Granulocyte-Colony Stimulating Factor (G-CSF) mobilizes stem cells for transplantation. G-CSF alters immune cells and cytokines, impacting transplant outcomes.

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In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
10:27

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

Published on: September 10, 2018

Area of Science:

  • Immunology
  • Hematology
  • Transplantation Medicine

Background:

  • Hematopoietic stem cell transplantation (HSCT) is a vital curative therapy for hematological and hereditary diseases.
  • Graft-versus-host disease (GVHD) is a significant complication and cause of mortality following allogeneic HSCT.
  • Peripheral blood HSCT, utilizing Granulocyte-Colony Stimulating Factor (G-CSF) for stem cell mobilization, is the predominant method.

Purpose of the Study:

  • To investigate the correlation between graft cell dose and clinical outcomes in HSCT.
  • To evaluate the immunologic changes induced by G-CSF in lymphocyte and cytokine profiles of allogeneic HSCT donors.

Main Methods:

  • Mobilization of HSC in twelve donors using G-CSF (16 microg/kg/day for 5 days).
  • Collection of Peripheral Blood (BPB), Mobilized Peripheral Blood (MPB), and apheresis mononuclear cells (G-MNC) samples.
  • Quantification of immune cells (CD19(+), CD3(+), CD4(+), CD8(+), NK, NKT, DC1, DC2) via flow cytometry and cytokine analysis (IL-4, IFNgamma, IL-2) using ELISA.

Main Results:

  • G-CSF significantly increased CD19(+), DC1, and DC2 cell populations in MPB compared to BPB.
  • Mobilized peripheral blood exhibited elevated Th2 cytokines (IL-4) and reduced Th1 cytokines (IFNgamma, IL-2).

Conclusions:

  • G-CSF administration induces significant alterations in immune cell populations and cytokine profiles.
  • Th1 and Th2 cytokine levels are crucial predictors of clinical outcomes in allogeneic peripheral blood HSCT.