Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

Published on: June 10, 2014

Multiplexed, high-throughput analysis of 3D microtissue suspensions.

Alice A Chen¹, Gregory H Underhill, Sangeeta N Bhatia

¹Massachusetts Institute of Technology, Cambridge, 02139, USA.

Integrative Biology : Quantitative Biosciences From Nano to Macro

|September 8, 2010

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineered microtissue systems for identifying the roles of Wnt and YAP signaling in hepatoblast differentiation and organization.

Materials today. Bio·2026

Same author

Electrical Stimulation Directs Formation of Perfused Vasculature in Engineered Tissues.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

A 3D In Vitro Model of the Human Hepatobiliary Junction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Synthetic control of implanted engineered liver tissue growth.

Science advances·2026

Same author

Exosome-niosome hybrid oxygen carrier for protection against acetaminophen-induced acute liver injury.

Biomaterials science·2026

Same author

Image-Guided Injectable Niche for Hepatocyte Transplantation.

bioRxiv : the preprint server for biology·2026

This study introduces a novel platform for analyzing three-dimensional (3D) microtissues, enabling high-throughput functional assessment of liver development and disease models. This method supports in vitro and in vivo studies for advanced regenerative medicine research.

Area of Science:

Biotechnology
Regenerative Medicine
Tissue Engineering

Background:

Three-dimensional (3D) tissue models are crucial for understanding biological structure/function relationships.
Current functional assessment methods for 3D tissue models are limited, hindering progress in regenerative medicine.

Purpose of the Study:

To develop and validate a platform for fabricating and quantitatively evaluating 3D microtissue suspensions.
To enable high-throughput, multiplexed analysis of 3D tissue biology in vitro and in vivo.

Main Methods:

Fabrication of 3D microtissues within polyethylene glycol hydrogels.
Application of multiparametric microtissue cytometry for quantitative analysis of large sample numbers (n≥ 10²-10³).
In vivo implantation and retrieval of 3D microtissues for assessment, alongside fluorescent labeling strategies for pooled experimentation.

More Related Videos

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Published on: April 25, 2025

Related Experiment Videos

Last Updated: Jun 9, 2026

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

Published on: June 10, 2014

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Published on: April 25, 2025

Main Results:

Demonstrated quantitative evaluation of microtissues representing various liver development and disease stages.
Enabled sorting-based enrichment of microtissue subsets for further study.
Identified synergistic effects of doxorubicin and BCL-XL siRNA knockdown in 3D hepatoma microtissues.

Conclusions:

The developed microtissue suspension platform offers broad utility for high-n, populational analysis of 3D tissue biology.
This approach facilitates advanced studies in regenerative medicine and drug discovery.
The platform supports both in vitro and in vivo investigations of complex biological systems.