Curcuminoids suppress the growth and induce apoptosis through caspase-3-dependent pathways in glioblastoma multiforme (GBM) 8401 cells
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Tzuu-Yuan Huang1, Tai-Hsin Tsai, Che-Wen Hsu
1Department of Neurosurgery, Tainan Sin-Lau Hospital, Tainan 71101, Taiwan.
Abstract:
Curcuminoids, natural plant components, have been recently shown to display antioxidant and anti-inflammatory activities. They also produce potent chemo-preventive action against several types of cancer. In the present study, the anti-proliferative and induced apoptosis effects of curcuminoids have been investigated in human brain glioblastoma multiforme (GBM) 8401 cells. Results indicated that curcuminoids have produced an inhibition of cell proliferation in a dose-dependent manner as dosage increased from 12.5 to 100 μM (n = 6) via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as well as activation of apoptosis in GBM 8401 cells. Both effects were observed to increase in proportion with the dose of curcuminoids. We have studied the mitochondrial membrane potential (ΔΨm), DNA fragmentation, caspase-3, caspase-8, and caspase-9 activation, and nuclear factor κB (NF-κB) transcriptional factor activity to analyze apoptosis in GBM 8401 cells. From these approaches, apoptosis was induced by curcuminoids in human brain GBM 8401 cells via mitochondria and a caspase-dependent pathway. The results observed with proliferation inhibition (y = 94.694e(-0.025x), R(2) = 0.9901, and n = 6) and apoptosis (y = 0.9789e(-0.0102x), R(2) = 0.99854, and n = 3) depend upon the amount of curcuminoid treatment in the cancer cells.
Insights
Curcuminoids inhibit human brain glioblastoma cell proliferation and induce apoptosis in a dose-dependent manner. These natural compounds activate apoptosis through mitochondria and caspase pathways, offering potential chemo-preventive effects.
Area of Science:
- Natural Products Chemistry
- Cancer Biology
- Cellular and Molecular Oncology
Background:
- Curcuminoids, derived from plants, exhibit antioxidant, anti-inflammatory, and chemo-preventive properties.
- Glioblastoma multiforme (GBM) is an aggressive form of brain cancer with limited treatment options.
Purpose of the Study:
- To investigate the anti-proliferative and apoptosis-inducing effects of curcuminoids on human glioblastoma multiforme (GBM) 8401 cells.
- To elucidate the molecular mechanisms underlying curcuminoid-induced apoptosis in GBM cells.
Main Methods:
- Cell proliferation was assessed using the MTT assay.
- Apoptosis was analyzed by evaluating mitochondrial membrane potential (ΔΨm), DNA fragmentation, and the activation of caspases (caspase-3, -8, -9) and nuclear factor κB (NF-κB).
Main Results:
- Curcuminoids demonstrated a dose-dependent inhibition of GBM 8401 cell proliferation.
- Curcuminoids significantly induced apoptosis in GBM 8401 cells, with effects increasing proportionally to the dose.
- Apoptosis was mediated through the mitochondrial pathway and involved caspase activation.
Conclusions:
- Curcuminoids possess significant anti-proliferative and apoptosis-inducing capabilities against human glioblastoma cells.
- The findings suggest curcuminoids may serve as a potential therapeutic agent for glioblastoma treatment.
- Further research into curcuminoid-based therapies for brain cancer is warranted.
Related Concept Videos
01:31The Intrinsic Apoptotic Pathway
01:24Caspases