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Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

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[Extended-spectrum beta-lactamase producing-enterobacteriaceae].

C Doit1, P Mariani-Kurkdjian, E Bingen

  • 1Service de bactériologie, Université Paris-Diderot, Hôpital Robert Debré, 48, Boulevard Sérurier, 75935 Paris cedex 19, France.

Archives De Pediatrie : Organe Officiel De La Societe Francaise De Pediatrie
|September 10, 2010
PubMed
Summary
This summary is machine-generated.

Extended-spectrum beta-lactamase (ESBL) producing E. coli infections are rising, causing urinary tract infections and rarely severe maternal or neonatal conditions. These ESBL strains remain susceptible to colimycin, carbapenems, and fosfomycin.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Extended-spectrum beta-lactamases (ESBLs) are enzymes conferring resistance to key antibiotics like cephalosporins and aztreonam.
  • Mobile genes encode ESBLs, with CTX-M, SHV, and TEM families being most prevalent.
  • ESBLs were initially identified in Klebsiella pneumoniae and subsequently became predominant in Escherichia coli.

Purpose of the Study:

  • To analyze the incidence and clinical impact of ESBL-producing E. coli at Robert Debré Hospital.
  • To identify common infections caused by these resistant strains.
  • To determine the susceptibility patterns of ESBL E. coli to available antibiotics.

Main Methods:

  • Retrospective analysis of patient data at Robert Debré Hospital.
  • Identification and characterization of ESBL-producing E. coli isolates.
  • Antimicrobial susceptibility testing.

Main Results:

  • A significant increase in patients with ESBL-producing E. coli infections observed since 2000.
  • Commonly associated with cystitis and pyelonephritis; rare cases of materno-foetal infections and neonatal meningitis reported.
  • Isolates demonstrated susceptibility to colimycin, carbapenems, and fosfomycin.

Conclusions:

  • ESBL-producing E. coli represent a growing challenge, primarily causing urinary tract infections.
  • Despite resistance to many antibiotics, effective treatment options like colimycin, carbapenems, and fosfomycin are available.
  • Continued surveillance and appropriate antibiotic selection are crucial for managing these infections.