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Electron microscopic studies on radiation-induced changes of cell surface negative charges
Z Somosy1, T Kubasova, O Csuka
1Frédéric Joliot-Curie, National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary.
Acta Physiologica Hungarica
|January 1, 1990
Summary
Ionizing radiation alters negatively charged cell surface sites in fibroblasts and melanoma cells, but not in leukemic cells. This study investigates cell membrane charge distribution changes post-irradiation.
Area of Science:
- Cell Biology
- Biophysics
Background:
- Cell surface charge is crucial for cellular functions.
- Understanding charge distribution aids in comprehending cell behavior and response to stimuli.
Purpose of the Study:
- To investigate the amount and distribution of negatively charged sites on human fibroblasts, B16 melanoma cells, and human lymphoid leukemic cells.
- To determine the impact of ionizing radiation on these negatively charged sites.
Main Methods:
- Comparative analysis of cell surface charge distribution using microscopy.
- Assessment of changes in negatively charged sites before and after ionizing radiation exposure.
Main Results:
- Non-irradiated fibroblasts and melanoma cells exhibited polarized distribution of negative charges.
- Human lymphoid leukemic cells showed a patched distribution of negative charges.
- Ionizing radiation altered the amount and distribution of negative charges on fibroblasts and melanoma cells, but not on leukemic cells.
Conclusions:
- Cellular response to ionizing radiation varies based on cell type.
- Leukemic cell membranes demonstrate a distinct topology of negative charges resistant to radiation-induced changes.