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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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MicroRNAs

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Related Experiment Video

Updated: Jun 9, 2026

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

MicroRNA activity in B lymphocytes.

Virginia G de Yébenes¹, Almudena R Ramiro

¹DNA Hypermutation and Cancer Group, Spanish National Cancer Research Center (CNIO), Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|September 10, 2010

Summary

This study identifies microRNAs (miRNAs) crucial for mature B cell function using a functional screening approach in primary mouse B cells. The findings highlight specific miRNAs that regulate B cell proliferation, apoptosis, and class switch recombination.

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Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Area of Science:

Immunology
Molecular Biology
Genetics

Background:

MicroRNAs (miRNAs) are key regulators of gene expression, influencing critical processes in B cell differentiation and function.
Previous research has established the role of miRNAs in various aspects of B cell biology.

Purpose of the Study:

To develop and apply a methodology for functional screening of a miRNA library.
To identify specific miRNAs that impact mature B cell function in primary mouse B cells.

Main Methods:

Construction of a comprehensive miRNA library.
Expression of individual miRNA clones in primary mouse spleen B cells.
Utilization of functional assays to assess B cell proliferation, apoptosis, and class switch recombination.

Main Results:

Successful establishment of a screening platform for miRNA function in B cells.
Identification of miRNAs that modulate key B cell functions.
Detailed characterization of the impact of specific miRNAs on B cell responses.

Conclusions:

This work provides a robust method for discovering functionally relevant miRNAs in B cells.
The identified miRNAs offer potential targets for modulating B cell responses in various immunological contexts.