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Updated: Jun 8, 2026

11:08
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides
1Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
Abstract:
A novel D-galactosyl-β1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Galβ1-3GalNAc), lacto-N-biose (Galβ1-3GlcNAc), and their derivatives.
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