Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antigen Processing Pathways01:31

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LRP4 is an entry receptor for multiple encephalitic alphaviruses.

Nature communications·2026
Same author

CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses.

bioRxiv : the preprint server for biology·2026
Same author

Protective human antibodies against Powassan virus.

Journal of virology·2026
Same author

Multiple LDLR family members act as entry receptors for yellow fever virus.

Nature·2025
Same author

LRP8 is an entry receptor for tick-borne encephalitis viruses.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Determinants of human versus mosquito cell entry by the Chikungunya virus envelope proteins.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 8, 2026

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

Basic and translational applications of engineered MHC class I proteins.

Ted H Hansen1, Janet M Connolly, Keith G Gould

  • 1Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA. hansen@pathology.wustl.edu

Trends in Immunology
|September 14, 2010
PubMed
Summary

Engineered single chain trimers (SCTs) offer versatile applications in diagnostics and therapeutics. These major histocompatibility complex (MHC) molecules are valuable tools for studying T cells, vaccines, and lymphocyte biology.

More Related Videos

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
11:17

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Related Experiment Videos

Last Updated: Jun 8, 2026

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
11:17

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Major histocompatibility complex (MHC) class I molecules are crucial for immune responses.
  • Single chain trimers (SCTs) are engineered MHC class I molecules incorporating peptide, β2 microglobulin, and heavy chain sequentially.
  • SCTs offer a stable and versatile format for presenting MHC-peptide complexes.

Purpose of the Study:

  • To review the recent and diverse applications of engineered single chain trimers (SCTs).
  • To highlight the utility of SCTs as diagnostic and therapeutic reagents.
  • To explore the potential of SCTs as probes for biological questions.

Main Methods:

  • Review of existing literature on SCT applications.
  • Analysis of diverse biological and immunological contexts where SCTs have been employed.
  • Discussion of engineering strategies for SCT development.

Main Results:

  • SCTs have enabled new approaches for enumerating disease-related T cells.
  • Applications include DNA vaccines and eliciting immune responses to pre-assembled complexes.
  • SCTs serve as unique probes for lymphocyte development and activation studies.

Conclusions:

  • Engineered SCTs represent a powerful platform with expanding applications in immunology and medicine.
  • Future advancements in SCT engineering and peptide identification will drive novel diagnostic and therapeutic strategies.
  • SCTs are valuable tools for fundamental research and clinical translation.