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Updated: Jun 8, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Survival of Lactobacillus rhamnosus strains in the upper gastrointestinal tract
Iole Pitino1, Cinzia Lucia Randazzo, Giuseppina Mandalari
1DOFATA, Dipartimento di Orto Floro Arboricoltura e Tecnologie Agro-alimentari, University of Catania, Catania, Italy.
Abstract:
In the present study six probiotic Lactobacillus rhamnosus strains were investigated for their ability to survive in the human upper gastrointestinal tract through a dynamic gastric model of digestion. MRS broth was used as delivery vehicle and survival was investigated during in vitro gastric and gastric plus duodenal digestion. Results highlighted that all tested strains showed good survival rate during both gastric and duodenal digestion. In particular, three strains exhibited a great survival showing a recovery percentage in the range between 117 and 276%. In agreement with survival data, high lactic acid production was detected for all strains, confirming their metabolic activity during digestion.
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