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Quantitative screening of EGF receptor-binding peptides by using a peptide library with multiple fluorescent amino

Mizuki Kitamatsu1, Takahiro Yamamoto, Midori Futami

  • 1Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University, Kita-ku, Okayama, Japan. kitamatu@cc.okayama-u.ac.jp

Bioorganic & Medicinal Chemistry Letters
|September 14, 2010
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...