Related Experiment Video
Updated: Jun 8, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
CG base pair recognition within DNA triple helices by modified N-methylpyrrolo-dC nucleosides
Simon R Gerrard1, Mastoura M Edrees, Imenne Bouamaied
1School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
None:
3-Aminophenyl-modified analogues of the bicyclic nucleoside N-methyl-3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one were synthesised and incorporated directly into triplex-forming oligonucleotides in order to utilise their extended hydrogen bonding motif for recognition of the CG base pair. All analogues demonstrated strong binding affinity and very good selectivity for CG from pH 6.2 to 7.0; a marked improvement on previous modifications.
Related Concept Videos
DNA Base Pairing
DNA Base Pairing
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Base-pairing and DNA Repair
Proofreading
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Proofreading

