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Live-cell imaging of cellular proteins by a strain-promoted azide-alkyne cycloaddition

Kimberly E Beatty1, John D Fisk, Brian P Smart

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

Chembiochem : a European Journal of Chemical Biology
|September 14, 2010
PubMed
Abstract

No abstract available in PubMed .

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...