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Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Generation of Monoclonal Antibodies Against Natural Products

Generation of Monoclonal Antibodies Against Natural Products

Published on: April 6, 2019

An optimized immunoaffinity fluorescent method for natural product target elucidation.

Wei-Luen Yu¹, Gianni Guizzunti, Timothy L Foley

¹Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, USA.

Journal of Natural Products

|September 15, 2010

Summary

A novel immunoaffinity fluorescent method enables parallel cellular and molecular studies for drug discovery. This technique aids in understanding small molecule mechanisms of action using a single probe.

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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

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Published on: November 2, 2009

Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Understanding small molecule mechanisms of action is crucial for drug discovery.
Existing methods may require separate assays for cellular and molecular analysis.

Purpose of the Study:

To develop and validate an optimized immunoaffinity fluorescent method.
To enable parallel studies at both cellular and molecular levels using a single probe.

Main Methods:

Immunoaffinity purification coupled with fluorescence detection.
Development of a single probe construct for dual-level analysis.
Analytical validation and application using glycyrrhetic acid as a model compound.

Main Results:

Demonstrated the viability of the optimized method.
Successfully applied the method to study glycyrrhetic acid.
Enabled parallel assessment of cellular and molecular effects.

Conclusions:

The developed method offers an efficient approach for small molecule mechanism of action studies.
This technique facilitates integrated analysis in drug discovery.
The method is robust and applicable to various small molecules.