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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Overview of quantitative LC-MS techniques for proteomics and activitomics
John F Timms1, Pedro R Cutillas
1Cancer Proteomics Laboratory, EGA Institute for Women's Health, University College London, London, UK.
Methods in Molecular Biology (Clifton, N.J.)
|September 15, 2010
Summary
Liquid chromatography-mass spectrometry (LC-MS) enables multiplex quantification of proteins, peptides, and metabolites. This technique is valuable for systems biology and understanding biochemical pathway activity.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Systems Biology
Background:
- Liquid chromatography-mass spectrometry (LC-MS) is a versatile analytical technique.
- It is widely used for the quantification of proteins and peptides.
- LC-MS also plays a role in systems biology research.
Purpose of the Study:
- To review analytical techniques utilizing LC-MS for multiplex quantification.
- To discuss the quantification of proteins, post-translational modifications, and metabolites.
- To highlight LC-MS applications in assessing biochemical pathway activity.
Main Methods:
- Review of analytical techniques based on LC-MS.
- Focus on methods enabling multiplex quantification.
- Application in proteomics, metabolomics, and post-translational modification analysis.
Main Results:
- LC-MS facilitates simultaneous quantification of multiple analytes.
- The technique allows for the measurement of proteins, peptides, metabolites, and post-translational modifications.
- Demonstrates utility in systems biology for pathway activity assessment.
Conclusions:
- LC-MS is a powerful tool for multiplex quantification in biological research.
- It enables comprehensive analysis of proteins, modifications, and metabolites.
- Essential for advancing systems biology and understanding cellular functions.
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