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Related Experiment Video

Updated: Jun 8, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantification of proteins by iTRAQ.

Richard D Unwin1

  • 1Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Manchester, UK.

Methods in Molecular Biology (Clifton, N.J.)
|September 15, 2010
PubMed
Summary

Isobaric tags for relative and absolute quantification (iTRAQ) enable precise protein quantification in proteomics. This method labels peptides, allowing for sensitive and accurate relative protein abundance measurements through reporter ion analysis.

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Protein relative quantification is crucial for proteomics.
  • Various methods exist, including isobaric tagging.

Purpose of the Study:

  • To describe the use of isobaric tags for relative and absolute quantification (iTRAQ).
  • To detail the procedure for labelling and analyzing peptide/protein samples using iTRAQ.

Main Methods:

  • Chemical tags (iTRAQ) attach to peptides via free amines.
  • Labeled samples are pooled and analyzed simultaneously using mass spectrometry.
  • Reporter ion intensity ratios indicate relative peptide proportions.

Main Results:

  • iTRAQ offers moderate sensitivity increase by summing signals from isobaric peptides.

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Related Experiment Videos

Last Updated: Jun 8, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
08:23

Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

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  • Peptide fragmentation releases tag-specific reporter ions.
  • Reporter ion ratios accurately reflect relative peptide abundance across samples.

    • Conclusions:

    • iTRAQ is an effective method for protein relative quantification in proteomics.
    • The technique enhances sensitivity and provides accurate relative abundance data.