Related Experiment Video
Updated: Jun 8, 2026
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Quantification of proteins by label-free LC-MS/MS
1Institute of Biotechnology, University of Cambridge, Cambridge, UK.
Methods in Molecular Biology (Clifton, N.J.)
|September 15, 2010
Summary
This study introduces a label-free, quantitative proteomic profiling protocol using LC-MS/MS. This method enhances statistical power for systems biology and biomarker discovery without pooling or labeling samples.
Area of Science:
- Proteomics
- Systems Biology
- Biomarker Discovery
Background:
- Quantitative proteomic profiling is crucial for systems biology and biomarker discovery.
- Increased statistical power in quantitative studies necessitates a high number of non-pooled samples.
- Existing methods may require sample pooling or labeling, potentially limiting data or introducing bias.
Purpose of the Study:
- To present a descriptive protocol for label-free quantitative proteomic profiling.
- To enable simultaneous quantitative and qualitative protein information acquisition.
- To address the need for high statistical power using non-pooled samples.
Main Methods:
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) based approach.
- Label-free quantitation strategy.
- Descriptive protocol development.
Main Results:
- The protocol allows for quantitative and qualitative protein analysis in a single study.
- It eliminates the need for sample pooling.
- It avoids the requirement for isotopic labeling of samples.
Conclusions:
- This label-free LC-MS/MS protocol provides a powerful tool for quantitative proteomics.
- It supports biomarker discovery and systems biology research by enabling analysis of non-pooled samples.
- The method offers both quantitative and qualitative insights, enhancing study robustness.
