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Updated: Jun 8, 2026

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms
Published on: January 11, 2015
Polar alkaloids from the Caribbean marine sponge Niphates digitalis
Erik L Regalado1, Judith Mendiola, Abilio Laguna
1Department of Chemistry, Center of Marine Bioproducts (CEBIMAR), Loma y 37, Alturas del Vedado, C.P. 10400 Havana, Cuba. erikluis18@gmail.com
Abstract:
A method involving flash chromatography, semi-preparative phenylhexyl RP HPLC-DAD-ELSD combined with analytic polar-RP HPLC-DAD, was applied to separate and purify six highly nitrogenated bases and a bicyclic amidine alkaloid, the major components of the marine sponge Niphates digitalis. Their structures were identified as 1,8-diazabicyclo[5.4.0]undec-7-ene (1), deoxycytidine (2), phenylalanine (3), adenosine (4), deoxyguanosine (5), adenine (6) and thymidine (7) on the basis of spectroscopic data analyses. This is the first report of these compounds in a marine sponge belonging to the Niphates genus and the first evidence of the presence of 1 from a natural source.
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