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Updated: Jun 8, 2026
Isolation of Preadipocytes from Broiler Chick Embryos
Published on: August 4, 2022
Chicken model of steroid-induced bone marrow adipogenesis using proteome analysis: a preliminary study
Sing Chung Li1, Ching Yu Lin, Tzong Fu Kuo
1Department of Radiology, School of Medicine, Taipei Medical University, Taipei 110, Taiwan, Republic of China. wp.chan@msa.hinet.net.
Background:
Steroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow. This may be a contributing factor in some forms of osteonecrosis. In this observational study, we aimed to determine the protein expression relating to steroid-induced adipogenesis of femoral bone marrow with use of a chicken model. We compared the histologic features of the femoral marrow of eight methylprednisolone (MP)-treated chickens with those of three control chickens and assessed differential proteins with 2-dimensional gel electrophoresis and differential proteins were identified by MALDI-TOF MS.
Results:
One MP-induced chicken died of overdose anesthesia. Methylprednisolone-induced proliferation of adipose tissue and new bone formation were found on histologic examination. In our study, 13 proteins in the control and MP-induced groups were differently expressed and nine protein spots showed marked threefold downregulation after 19 weeks of MP treatment. These were serum amyloid P-component precursor, zinc finger protein 28, endothelial zinc finger protein 71, T-box transcription factor 3, cyclin-dependent kinase inhibitor 1, myosin 1D, dimethylaniline monooxygenase, and two uncharacterized proteins.
Conclusions:
Proteomic profiling can be a useful dynamic approach for detecting protein expression in MP-induced adipogenesis of the femur in chickens.
