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Updated: Jun 8, 2026
09:41
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Cyclin G2 regulates adipogenesis through PPAR gamma coactivation.
Victor Aguilar1, Jean-Sébastien Annicotte, Xavier Escote
1Institut de Recherche en Cancérologie de Montpellier, CRLC Val d'Aurelle, Parc Euromédecine, F-34298, Montpellier Cedex 5, France.
Endocrinology
|September 17, 2010
Summary
Cyclin G2 promotes adipogenesis by directly interacting with peroxisome proliferator-activated receptor γ (PPARγ), a key regulator of fat cell differentiation. This novel finding highlights cyclin G2
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Cell cycle regulators are crucial for adipocyte differentiation.
- Cyclin G2 is an unconventional cyclin upregulated during growth inhibition or apoptosis.
Purpose of the Study:
- To investigate the role of cyclin G2 in adipogenesis.
- To elucidate the mechanism by which cyclin G2 regulates adipocyte differentiation.
Main Methods:
- 3T3-F442A cell line for adipogenesis studies.
- Western blot and quantitative PCR for gene and protein expression analysis.
- Chromatin immunoprecipitation and luciferase reporter assays to determine molecular interactions and transcriptional activity.
Main Results:
- Cyclin G2 expression is upregulated during adipocyte differentiation.
- Overexpression of cyclin G2 enhances adipogenesis.
- Cyclin G2 directly binds to and coactivates peroxisome proliferator-activated receptor γ (PPARγ).
- Cyclin G2 is present at the PPARγ-responsive element of the aP2 promoter and enhances PPARγ transcriptional activity.
- Cyclin G2 expression is increased in mice fed a high-fat diet.
Conclusions:
- Cyclin G2 acts as a novel positive regulator of adipogenesis.
- The mechanism involves direct interaction and coactivation of PPARγ.
- Cyclin G2 plays a significant role in adipocyte differentiation and may be implicated in diet-induced obesity.
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