[Uracil, contained in DNA, can direct the correction of mispaired nucleotides in human cells]

The mismatch correction has been studied in human cells and presented in this paper. In the study the experimental model with half-containing hetroduplex (-1 residue in the polylinker region of lac gene) M13 DNA has been used. M 13 DNA was isolated from human cells 24 hours after transfection and transformed into ung+ and ung- cells of Escherichia coli. The percentage of lac- colonies (formed due to frameshift mutation in the lac gene) was analyzed. The increased percent of lac- mutations after human transfection indicated that DNA-uracil can polarize mismatch correction in human cells.