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Updated: Jun 8, 2026
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Corneal Donor Tissue Preparation for Endothelial Keratoplasty
Published on: June 12, 2012
Tissue complications during endothelial keratoplasty.
1Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. dbg@comcast.net
Cornea
|September 18, 2010
Summary
Inseparable corneal lamellae during Descemet stripping automated endothelial keratoplasty (DSAEK) preparation can be caused by cutting errors or premature separation. Careful donor cornea inspection can prevent issues and allow successful DSAEK.
Area of Science:
- Ophthalmology
- Corneal Surgery
- Tissue Engineering
Background:
- Descemet stripping automated endothelial keratoplasty (DSAEK) is a common procedure for treating endothelial dysfunction.
- Proper preparation of donor corneal tissue is crucial for successful DSAEK outcomes.
Observation:
- Six cases of apparently inseparable corneal lamellae during DSAEK tissue preparation were reported.
- These cases involved issues during intraoperative handling and eye bank precut tissue.
Findings:
- Inadequate lamellar cuts or premature separation of corneal layers were identified as primary causes.
- Surgery was aborted in four cases due to inseparable lamellae; one case involved successful transplantation after identifying a free anterior cap.
Implications:
- Thorough pre-operative inspection of donor corneas under magnification is essential to identify potential issues.
- Understanding these complications can improve DSAEK tissue preparation protocols and surgical success rates.
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