Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Healing II: Complications

Healing II: Complications

Complications during healing arise when tissue repair is altered by local or systemic factors. These changes involve abnormal collagen deposition, altered biomechanics, and reduced vascular supply, impairing restoration of normal structure and function.Loss of FunctionScar tissue differs significantly from the original tissue it replaces. In the skin, fibrosis lacks adnexal structures such as hair follicles, sebaceous glands, and sweat glands. Their absence reduces tactile sensitivity, impairs...

Tissue Transplantation

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications

Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications

Peritoneal dialysis (PD) is a medical process that removes waste products and excess fluid from the body using the peritoneal membrane as a natural filter.Peritoneal Dialysis MethodsSeveral methods can be used for peritoneal dialysis, including Acute Intermittent Peritoneal Dialysis, Continuous Ambulatory Peritoneal Dialysis, and Automated Peritoneal Dialysis, also known as Continuous Cyclic Peritoneal Dialysis.Acute Intermittent Peritoneal Dialysis (AIPD) is used for patients with uremic...

Aneurysm III: Interprofessional Care

Aneurysm III: Interprofessional Care

Aneurysm management involves either conservative medical therapy or surgical intervention, depending on the size and symptoms of the aneurysm. Conservative management is generally reserved for smaller, asymptomatic aneurysms, while larger or symptomatic aneurysms often necessitate surgical repair.Conservative Medical TherapyFor small, asymptomatic aneurysms, particularly abdominal aortic aneurysms (AAA) less than 5.5 centimeters in diameter, conservative medical therapy is recommended. This...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Amniotic Membrane Grafts for Dry Eye.

Ophthalmology·2025

Same author

Cataract Surgery in the Medicare Merit-Based Incentive Payment System: Episode-Based Cost Measure Development and Evaluation.

Ophthalmology science·2023

Same author

Is There a Future for Telehealth in Ophthalmology?

JAMA ophthalmology·2022

Same author

The Promise and Perils of Biosimilars in Ophthalmology.

Ophthalmology. Retina·2022

Same author

Re: Asahi et al.: Medicare reimbursement trends for vitreoretinal procedures: 2000 to 2020 (Ophthalmology Retina. 2022;6:326-328).

Ophthalmology. Retina·2022

Same author

Trends in Early Graft Failure Leading to Regrafting After Endothelial Keratoplasty in the United States.

Cornea·2021

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Corneal Donor Tissue Preparation for Endothelial Keratoplasty

Corneal Donor Tissue Preparation for Endothelial Keratoplasty

Published on: June 12, 2012

Tissue complications during endothelial keratoplasty.

David B Glasser¹

¹Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. dbg@comcast.net

|September 18, 2010

Summary

Inseparable corneal lamellae during Descemet stripping automated endothelial keratoplasty (DSAEK) preparation can be caused by cutting errors or premature separation. Careful donor cornea inspection can prevent issues and allow successful DSAEK.

More Related Videos

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

Published on: September 17, 2014

An “All-laser” Endothelial Transplant

An “All-laser” Endothelial Transplant

Published on: July 6, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

Corneal Donor Tissue Preparation for Endothelial Keratoplasty

Corneal Donor Tissue Preparation for Endothelial Keratoplasty

Published on: June 12, 2012

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

Published on: September 17, 2014

An “All-laser” Endothelial Transplant

An “All-laser” Endothelial Transplant

Published on: July 6, 2015

Area of Science:

Ophthalmology
Corneal Surgery
Tissue Engineering

Background:

Descemet stripping automated endothelial keratoplasty (DSAEK) is a common procedure for treating endothelial dysfunction.
Proper preparation of donor corneal tissue is crucial for successful DSAEK outcomes.

Observation:

Six cases of apparently inseparable corneal lamellae during DSAEK tissue preparation were reported.
These cases involved issues during intraoperative handling and eye bank precut tissue.

Findings:

Inadequate lamellar cuts or premature separation of corneal layers were identified as primary causes.
Surgery was aborted in four cases due to inseparable lamellae; one case involved successful transplantation after identifying a free anterior cap.

Implications:

Thorough pre-operative inspection of donor corneas under magnification is essential to identify potential issues.
Understanding these complications can improve DSAEK tissue preparation protocols and surgical success rates.