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Updated: Jun 8, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
Published on: May 3, 2015
Fabrication, calibration and evaluation of a phosphate ion-selective microelectrode
1University of Cincinnati, 12716 Ginger Wood Lane, Clarksburg, MD 20871, USA. johnwang1974@gmail.com
Abstract:
To conduct the micro-environment study of flocs in an enhanced biological phosphorus removal (EBPR) process, a phosphate ion-selective microelectrode was developed. The cobalt-based microelectrodes have tip diameters of 5-20μm and respond to all the three forms of phosphate ions, namely, H(2)PO(4)(-), HPO(4)(2-), and PO(4)(3-). The calibration curve at pH 7.5 had a slope of 31.5mV per decade change of concentration and a R(2) value of 0.99. Other characteristics of this microelectrode, such as response time, interferences from pH, ion strength, DO and other anions were also evaluated.

