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Related Experiment Video

Updated: Jun 8, 2026

Applications for Open Source Microplate-Compatible Illumination Panels

Applications for Open Source Microplate-Compatible Illumination Panels

Published on: October 3, 2019

Capillary wells microplate with side optical access.

Han Yen Tan¹, Tuck Wah Ng, Adrian Neild

¹Laboratory for Optics, Acoustics, & Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, Australia.

Journal of Biomolecular Screening

|September 21, 2010

Summary

This summary is machine-generated.

Bubbles and pipetting errors in microplate assays lead to inaccurate fluorescence measurements. This study presents a novel capillary well microplate design with side viewing to detect these errors, ensuring reliable high-throughput screening data.

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Area of Science:

Biotechnology
Assay Development
Analytical Chemistry

Background:

Bubbles in microplate wells cause significant inaccuracies in fluorescence measurements.
Pipetting errors can lead to misleading data and incorrect interpretations, especially in high-throughput screening (HTS).
Accurate data is crucial for reliable results in biological and chemical assays.

Purpose of the Study:

To develop and demonstrate a method for detecting bubbles and pipetting errors in microplate-based assays.
To improve the accuracy and reliability of fluorescence measurements in microplate screening.
To adapt the capillary wells microplate approach for side-viewing detection capabilities.

Main Methods:

An adapted capillary wells microplate design enabling side viewing was developed.
A prototype system was created to detect bubbles and pipetting errors.
The system was tested during actual assay runs to validate its performance.

Main Results:

The adapted capillary wells microplate design allows for effective side viewing.
The prototype successfully detected bubbles and pipetting errors in real-time during assays.
The developed method ensures greater accuracy in fluorescence measurements.

Conclusions:

The novel capillary well microplate design with side viewing is effective in detecting assay interferences.
This approach enhances the reliability of data obtained from high-throughput screening.
The technology offers a solution to common error sources in microplate-based assays.