Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Severity and Outcomes of SARS-CoV-2 Reinfection Compared with Primary Infection: A Systematic Review and Meta-Analysis.

International journal of environmental research and public health·2023

Same author

Mechanism of Tao Hong Decoction in the treatment of atherosclerosis based on network pharmacology and experimental validation.

Frontiers in cardiovascular medicine·2023

Same author

Recognition Site Modifiable Macrocycle: Synthesis, Functional Group Variation and Structural Inspection.

Molecules (Basel, Switzerland)·2023

Same author

Global Epidemiological Features of Human Monkeypox Cases and Their Associations With Social-Economic Level and International Travel Arrivals: A Systematic Review and Ecological Study.

International journal of public health·2023

Same author

Hydroxysafflor yellow A regulates lymphangiogenesis and inflammation via the inhibition of PI3K on regulating AKT/mTOR and NF-κB pathway in macrophages to reduce atherosclerosis in ApoE-/- mice.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2023

Same author

A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate.

Carbohydrate polymers·2023

Same journal

Rapid Evolution of Expression Levels in Hepatocellular Carcinoma.

International journal of computational biology and drug design·2021

Same journal

Identifying the dynamic gene regulatory network during latent HIV-1 reactivation using high-dimensional ordinary differential equations.

International journal of computational biology and drug design·2021

Same journal

PATH: An interactive web platform for analysis of time-course high-dimensional genomic data.

International journal of computational biology and drug design·2021

Same journal

Modelling of hypoxia gene expression for three different cancer cell lines.

International journal of computational biology and drug design·2020

Same journal

Brain-wide structural connectivity alterations under the control of Alzheimer risk genes.

International journal of computational biology and drug design·2020

Same journal

Native State of Complement Protein C3d Analysed via Hydrogen Exchange and Conformational Sampling.

International journal of computational biology and drug design·2019

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

A multi-view approach to cDNA micro-array analysis.

Bachar Zineddin¹, Zidong Wang, Yong Shi

¹Department of Information Systems and Computing, Brunel University, Uxbridge, Middlesex, UK. Bachar.Zineddin@brunel.ac.uk

International Journal of Computational Biology and Drug Design

|September 21, 2010

Summary

This summary is machine-generated.

This study enhances microarray image analysis by clarifying gene expression data. The Image Transformation Engine combined with the CLD filter offers superior performance for gene interaction studies.

More Related Videos

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Related Experiment Videos

Last Updated: Jun 8, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Area of Science:

Bioinformatics
Genomics
Image Analysis

Background:

Microarray technology allows simultaneous study of thousands of genes.
Understanding gene interaction and regulation is crucial in biology.
Efficient analysis of microarray image data is essential for biological discovery.

Purpose of the Study:

To improve the unsupervised analysis of microarray image data.
To clarify the feature space of microarray images.
To identify optimal image processing techniques for gene expression analysis.

Main Methods:

Investigation of the Image Transformation Engine (ITE) with various filters.
Application of proposed methods to real-world cDNA microarray images.
Utilizing the MatCNN toolbox for image segmentation.
Quantitative comparison of different filters' performance.

Main Results:

The Image Transformation Engine (ITE) was evaluated for its effectiveness.
Different filters were quantitatively compared in conjunction with the ITE.
The CLD filter demonstrated superior performance when used with the ITE.

Conclusions:

The CLD filter is the optimal choice for application with the Image Transformation Engine in microarray image analysis.
The proposed methods enhance the clarity of gene expression data.
This work contributes to more accurate gene interaction and regulation studies.