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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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ChemMatrix(®) for complex peptides and combinatorial chemistry.

Yésica García-Ramos1, Marta Paradís-Bas, Judit Tulla-Puche

  • 1Institute for Research in Biomedicine, Barcelona Science Park, Baldiri Reixac 10, Barcelona, Spain.

Journal of Peptide Science : an Official Publication of the European Peptide Society
|September 21, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces CM resin, a stable, user-friendly PEG-based material for peptide and protein synthesis. Its high loading capacity enables efficient preparation of complex molecules and microreactor applications.

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Area of Science:

  • Polymer Chemistry
  • Organic Synthesis
  • Biochemistry

Background:

  • Polyethylene glycol (PEG)-based resins are crucial in solid-phase synthesis.
  • Existing PEG resins have limitations in chemical stability and loading capacity.
  • Polystyrene (PS) resins are commonly used but less suitable for certain hydrophobic peptides.

Purpose of the Study:

  • To evaluate the performance of a novel PEG-based resin, CM resin, for peptide and protein synthesis.
  • To compare CM resin with existing PEG and PS resins.
  • To explore the applications of CM resin in combinatorial chemistry.

Main Methods:

  • Synthesis of peptides, including hydrophobic poly-Arg peptides.
  • Synthesis of small proteins, such as chemokine RANTES, using CM resin and ψPros.
  • On-bead screening in biocompatible solvents.
  • Utilizing a quarter of a bead as a microreactor for HPLC and MALDI-TOF analysis.

Main Results:

  • CM resin demonstrates high chemical stability due to its PEG-based structure with primary ether bonds.
  • It offers user-friendly handling and good loading capacity compared to other PEG resins.
  • CM resin shows superior performance for synthesizing hydrophobic, highly structured poly-Arg peptides.
  • Successful synthesis of small proteins like RANTES was achieved.
  • The resin swells well in water, facilitating on-bead screening.
  • High loading allows for microreactor applications in HPLC and MALDI-TOF analysis.

Conclusions:

  • CM resin is a highly stable and effective PEG-based resin for complex peptide and protein synthesis.
  • Its properties make it advantageous over traditional PS resins and other PEG resins for specific applications.
  • CM resin expands the utility of PEG-based resins in combinatorial chemistry and analytical techniques.