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Hormonal modulation of hepatic plasma membrane lactate transport in cultured rat hepatocytes
H K Metcalfe1, R D Cohen, J P Monson
1Cellular Mechanisms Group, London Hospital Medical College, U.K.
Abstract:
Hormonal modulation of hepatic plasma membrane lactate transport was studied in primary cultures of isolated hepatocytes from fed rats to examine the mechanism for the known enhancement of lactate transport in starvation and diabetes. Total cellular lactate entry was increased by 14% in the presence of dexamethasone; this was accounted for by an approximately 40% increase in the carrier-mediated component of entry with no effect on diffusion. A trend of similar magnitude was evident with glucagon. The effects of dexamethasone and glucagon on lactate transport constitute an additional potential mechanism for enhancement of gluconeogenesis by these hormones.