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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: Jun 8, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

A method for small molecule microarray-based screening for the rapid discovery of affinity-based probes.

Haibin Shi1, Mahesh Uttamchandani, Shao Q Yao

  • 1The NUS MedChem Program of the Office of Life Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel small molecule microarray (SMM) method for rapidly identifying affinity-based probes (AfBPs). This approach efficiently discovers specific and potent AfBPs for enzymes like gamma-secretase.

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Drug Discovery

Background:

  • Developing affinity-based probes (AfBPs) is crucial for studying enzyme function and identifying inhibitors.
  • Traditional methods for AfBP identification can be time-consuming and labor-intensive.

Purpose of the Study:

  • To present a new high-throughput method for identifying AfBPs using small molecule microarrays (SMMs).
  • To demonstrate the utility of SMMs for discovering specific and potent AfBPs against gamma-secretase.

Main Methods:

  • Generation of a hydroxylethylene-based small molecule library via solid-phase combinatorial synthesis.
  • Biotinylation of the library for immobilization on avidin-coated slides.
  • Screening of the library against gamma-secretase (purified protein and cell lysates) using the SMM platform.

Main Results:

  • Identification of several specific and potent AfBPs for gamma-secretase based on binding profiles.
  • Demonstration of the SMM platform's ability to sensitively detect activity-based binding interactions.
  • Successful redesign of identified binders into functional AfBPs.

Conclusions:

  • The SMM approach provides a rapid and efficient method for high-throughput identification of AfBPs.
  • This platform facilitates the discovery of small molecule binders and inhibitors for enzymes, including aspartic proteases.
  • The developed AfBPs offer valuable tools for gamma-secretase research.