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Updated: Jun 8, 2026

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
Published on: February 4, 2017
Tetrazine-trans-cyclooctene ligation for the rapid construction of 18F labeled probes
Zibo Li1, Hancheng Cai, Matthew Hassink
1Molecular Imaging Center, Department of Radiology, University of Southern California, Los Angeles 90033, USA. ziboli@usc.edu
Abstract:
A radiolabeling method for bioconjugation based on the Diels-Alder reaction between 3,6-diaryl-s-tetrazines and an (18)F-labeled trans-cyclooctene is described. The reaction proceeds with exceptionally fast rates, making it an effective conjugation method within seconds at low micromolar concentrations.
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