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Updated: Jun 8, 2026

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

Supramolecular hydrogel-based protein and chemosensor array.

Masato Ikeda¹, Rika Ochi, Itaru Hamachi

¹Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan.

|September 24, 2010

Summary

This summary is machine-generated.

Supramolecular hydrogels offer a novel matrix for immobilizing biomolecules and sensors, enabling high-throughput assays. This material preserves substance activity and facilitates signal detection for molecular recognition and enzyme activity monitoring.

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Last Updated: Jun 8, 2026

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

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Published on: August 26, 2013

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Synthesis of an Intein-mediated Artificial Protein Hydrogel

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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Area of Science:

Biomaterials Science
Nanotechnology
Analytical Chemistry

Background:

Protein and sensor arrays are vital for high-throughput biological and environmental assays.
Developing versatile matrices is key to immobilizing diverse assay components while maintaining their function.

Purpose of the Study:

To introduce supramolecular hydrogels as a unique matrix for creating arrays of biomolecules and sensors.
To demonstrate the hydrogel's capability to retain the activity of immobilized substances and enable various detection methods.

Main Methods:

Utilizing a 3D supramolecular nanofiber network hydrogel structure.
Immobilizing proteins, peptides, substrates, chemosensors, and mesoporous silica particles within the hydrogel matrix.
Employing hydrophobic entrapment within nanofibers for developing read-out systems like fluorescence enhancement and FRET.

Main Results:

The semi-wet hydrogel environment successfully entrapped various substances, preserving their activity and function.
Hydrophobic interiors of nanofibers enabled reversible entrapment of hydrophobic molecules.
Demonstrated potential for monitoring molecular recognition and enzyme activity through fluorescence-based read-out systems.

Conclusions:

Supramolecular hydrogels provide a versatile and effective matrix for constructing functional arrays.
The unique properties of these hydrogels support the development of advanced diagnostic and monitoring tools.
This approach enhances the capabilities of high-throughput assays for diverse applications.