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Updated: Jun 8, 2026

Comparing the Affinity of GTPase-binding Proteins using Competition Assays
Published on: October 8, 2015
Modelling the effects of inhibitors of guanine nucleotide synthesis: implications for studies of cellular
1Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK. g.thomas@ucl.ac.uk
Abstract:
Mizoribine induces the differentiation of promyelocytes by an unknown mechanism that relies on compromised guanine nucleotide synthesis. I have found that mizoribine also perturbs adenosine nucleotide levels in HL-60 promyelocytes, particularly ATP. To reconcile these observations with the known actions of mizoribine I have adapted an existing model of human purine metabolism composed as an S-system familiar from Biochemical Systems Theory. Mizoribine's actions were then simulated and compared with experimental data.
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