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Updated: Jun 8, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Aberrant methylation of tumor suppressor genes in patients with refractory anemia with ring sideroblasts
Ana Valencia1, José Cervera, Esperanza Such
1Department of Hematology, Hospital Universitario La Fe, Avda. Campanar 21, 46009 Valencia, Spain. valencia ana@gva.es
Abstract:
This study evaluates the incidence and prognostic impact of aberrant methylation of 25 tumor suppressor genes in 40 patients with RARS, a MDS subtype, by methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) assay. Methylation of at least one gene was detected in 18 patients (45%). The genes methylated were CDKN2B (20%), RASSF1 (18%), RARB (10%), CDH13 (7.5%) and FHIT (5%). Patients with at least one methylated gene had a significantly shorter OS than patients without methylated genes. Aberrant methylation is a frequent event in patients with RARS as in patients with high-risk MDS appears to confer a worse prognosis.
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