Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 8, 2026

Preparation of Functional Silica Using a Bioinspired Method
08:04

Preparation of Functional Silica Using a Bioinspired Method

Published on: August 1, 2018

Enzyme stabilization via bio-templated silicification reactions.

Glenn R Johnson1, Heather R Luckarift

  • 1Microbiology and Applied Biochemistry, Air Force Research Laboratory, Tyndall Air Force Base, Panama, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 25, 2010
PubMed
Summary

This study presents a novel silica sol-gel method for rapid enzyme entrapment, enhancing biocatalyst stability and activity. This biomimetic approach simplifies the creation of solid-phase enzymes for diverse biotechnology applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biotechnology to reduce logistics burden and promote environmental stewardship for Air Force civil engineering requirements.

Biotechnology advances·2023
Same author

Producing methane, methanol and electricity from organic waste of fermentation reaction using novel microbes.

Bioresource technology·2018
Same author

Simultaneous hydrolysis and fermentation of unprocessed food waste into ethanol using thermophilic anaerobic bacteria.

Bioresource technology·2017
Same author

Enzyme Stabilization via Bio-Templated Silicification Reactions.

Methods in molecular biology (Clifton, N.J.)·2016
Same author

Modification of carbon nanotube electrodes with 1-pyrenebutanoic acid, succinimidyl ester for enhanced bioelectrocatalysis.

Methods in molecular biology (Clifton, N.J.)·2013
Same author

Immobilization of whole cells by chemical vapor deposition of silica.

Methods in molecular biology (Clifton, N.J.)·2013

Area of Science:

  • Biochemistry
  • Materials Science
  • Biotechnology

Background:

  • Enzyme immobilization in solid-phase materials is crucial for practical applications, offering enhanced stability and simplified integration compared to soluble enzymes.
  • Current methods for enzyme entrapment can be time-consuming and may impact enzyme activity.
  • Developing efficient and stable solid-phase biocatalysts is essential for advancements in various biotechnological fields.

Purpose of the Study:

  • To develop a rapid and effective method for enzyme entrapment using a silica sol-gel process.
  • To demonstrate the ability of oligopeptides and proteins to mediate silica biomineralization for co-encapsulation.
  • To create stable, solid-phase biocatalysts with retained enzymatic activity.

Main Methods:

More Related Videos

Preparation of Macroporous Epitaxial Quartz Films on Silicon by Chemical Solution Deposition
07:37

Preparation of Macroporous Epitaxial Quartz Films on Silicon by Chemical Solution Deposition

Published on: December 21, 2015

High Temperature Fabrication of Nanostructured Yttria-Stabilized-Zirconia (YSZ) Scaffolds by In Situ Carbon Templating Xerogels
07:13

High Temperature Fabrication of Nanostructured Yttria-Stabilized-Zirconia (YSZ) Scaffolds by In Situ Carbon Templating Xerogels

Published on: April 16, 2017

Related Experiment Videos

Last Updated: Jun 8, 2026

Preparation of Functional Silica Using a Bioinspired Method
08:04

Preparation of Functional Silica Using a Bioinspired Method

Published on: August 1, 2018

Preparation of Macroporous Epitaxial Quartz Films on Silicon by Chemical Solution Deposition
07:37

Preparation of Macroporous Epitaxial Quartz Films on Silicon by Chemical Solution Deposition

Published on: December 21, 2015

High Temperature Fabrication of Nanostructured Yttria-Stabilized-Zirconia (YSZ) Scaffolds by In Situ Carbon Templating Xerogels
07:13

High Temperature Fabrication of Nanostructured Yttria-Stabilized-Zirconia (YSZ) Scaffolds by In Situ Carbon Templating Xerogels

Published on: April 16, 2017

  • Utilized a silica sol-gel process inspired by biomineralization mechanisms for protein immobilization.
  • Employed oligopeptides and selected proteins to initiate and control silica formation.
  • Co-encapsulated target proteins within the silica matrix during the rapid sol-gel reaction.

    • Main Results:

    • Achieved effective enzyme entrapment within minutes using the biomineralization-based silica sol-gel process.
    • Demonstrated high protein binding capacity of the resulting silica material.
    • Confirmed retention of catalytic activity in the immobilized enzymes.

    Conclusions:

    • The described method provides a simple, rapid, and effective approach for creating stable, solid-phase biocatalysts.
    • This technique enables the integration of enzymes into various applications such as sensors, synthetic processes, and energy conversion materials.
    • The biomineralization-inspired silica sol-gel process offers a versatile platform for enzyme immobilization in biotechnology.