Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Protein blotting: ten years on.

D R Harper¹, M L Kit, H O Kangro

¹Virology Department, St. Bartholomew's Hospital, London, U.K.

Journal of Virological Methods

|October 1, 1990

Summary

Western blotting, a protein analysis technique, has evolved since its 1979 inception. This review covers its historical changes and current methods for antibody-based protein detection in research and clinical applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm formation.

Applied and environmental microbiology·2014

Same author

Phage therapy: delivering on the promise.

Therapeutic delivery·2012

Same author

Bacteriophages for the treatment of Pseudomonas aeruginosa infections.

Journal of applied microbiology·2011

Same author

Determination of Viral Infectivity : HSV, VZV, and CMV.

Methods in molecular medicine·2011

Same author

Bacteriophage applications: where are we now?

Letters in applied microbiology·2010

Same author

A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy.

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2009

Area of Science:

Biochemistry
Molecular Biology
Immunology

Background:

Protein blotting, specifically Western blotting, emerged from nucleic acid techniques in 1979.
The technique became widely known as 'Western' blotting in 1981.
It enables electrophoresed proteins to be analyzed, primarily through reactions with antibodies or antisera.

Purpose of the Study:

To review the historical evolution of protein blotting techniques.
To summarize the current methodologies employed in Western blotting.
To highlight the diverse research and clinical applications of protein detection via blotting.

Main Methods:

Review of historical literature and seminal publications on protein blotting.
Analysis of modifications and advancements in Western blotting protocols over time.
Compilation of current standard operating procedures and emerging techniques.

Main Results:

The fundamental principles of protein blotting remain consistent despite numerous technical advancements.
Western blotting is a versatile tool with significant clinical and research utility.
The technique facilitates specific protein identification and quantification through antibody-based detection.

Conclusions:

Protein blotting techniques have undergone significant evolution since their introduction.
Current Western blotting methods offer enhanced sensitivity and specificity for protein analysis.
The continued development of blotting techniques supports ongoing advancements in biomedical research and diagnostics.

Related Experiment Videos