Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-resolution aberration-free imaging through GRIN fiber using speckle illumination and compressive sensing.

Optics letters·2026
Same author

Evaluation of thermochemical characteristics of salts with pentazenium cation.

Journal of molecular modeling·2025
Same author

Near-infrared optical thermometry of nickel color centers in diamond.

Optics letters·2025
Same author

Thermal and Quantum Barrier Passage as Potential-Driven Markovian Dynamics.

The journal of physical chemistry. B·2023
Same author

Sub-cycle pulse revealed with carrier-envelope phase control of soliton self-compression in anti-resonant hollow-core fiber.

Optics letters·2023
Same author

Enhanced coherent transition radiation from midinfrared-laser-driven microplasmas.

Scientific reports·2022
Same journal

Tension on dsDNA bound to ssDNA-RecA filaments may play an important role in driving efficient and accurate homology recognition and strand exchange.

Physical review. E, Statistical, nonlinear, and soft matter physics·2016
Same journal

Publisher's Note: Amplitude-phase coupling drives chimera states in globally coupled laser networks [Phys. Rev. E 91, 040901(R) (2015)].

Physical review. E, Statistical, nonlinear, and soft matter physics·2016
Same journal

Erratum: Shapes of sedimenting soft elastic capsules in a viscous fluid [Phys. Rev. E 92, 033003 (2015)].

Physical review. E, Statistical, nonlinear, and soft matter physics·2016
Same journal

Erratum: Attenuation of excitation decay rate due to collective effect [Phys. Rev. E 90, 022142 (2014)].

Physical review. E, Statistical, nonlinear, and soft matter physics·2016
Same journal

Publisher's Note: Role of connectivity and fluctuations in the nucleation of calcium waves in cardiac cells [Phys. Rev. E 92, 052715 (2015)].

Physical review. E, Statistical, nonlinear, and soft matter physics·2016
Same journal

Publisher's Note: Lattice Boltzmann approach for complex nonequilibrium flows [Phys. Rev. E 92, 043308 (2015)].

Physical review. E, Statistical, nonlinear, and soft matter physics·2016
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Ionization penalty in nonlinear optical bioimaging.

A A Voronin1, A M Zheltikov

  • 1Physics Department, International Laser Center, M. V. Lomonosov Moscow State University, Vorob'evy gory, Moscow 119992, Russia.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|September 28, 2010
PubMed
Summary
This summary is machine-generated.

Nonlinear optical imaging is noninvasive when laser field intensities exceed 1 TW/cm2. This threshold ensures minimal free electron generation during laser-tissue interactions, crucial for biological imaging safety.

More Related Videos

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

Related Experiment Videos

Last Updated: Jun 8, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

Area of Science:

  • Biomedical Optics
  • Laser-Tissue Interactions
  • Nonlinear Optics

Background:

  • Nonlinear optical imaging offers high resolution for biological samples.
  • Quantifying noninvasiveness is crucial for safe in vivo applications.
  • Laser-tissue interactions can lead to photodamage via free electron generation.

Purpose of the Study:

  • To quantify the noninvasiveness of nonlinear optical imaging techniques.
  • To determine the threshold laser field intensity for safe imaging.
  • To analyze the impact of laser parameters on tissue ionization.

Main Methods:

  • Quantified noninvasiveness by calculating the ratio of free electrons generated to nonlinear optical signal photons.
  • Investigated various biomarker dyes and bioactivity reporter proteins.
  • Derived analytical expressions for ionization penalty and critical pulse repetition rate.

Main Results:

  • The free electron generation ratio approaches unity at field intensities above 1 TW/cm2 for diverse biomarkers.
  • This critical ratio indicates a balance between signal generation and ionization.
  • Analytical expressions were derived for few-cycle laser pulses.

Conclusions:

  • Nonlinear optical imaging can be considered noninvasive above 1 TW/cm2 field intensity.
  • The findings provide a quantitative metric for assessing imaging safety.
  • This research aids in optimizing laser parameters for minimally invasive bioimaging.